FastQCFastQC Report
Mon 27 Feb 2023
SRR3127827.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127827.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748923
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110530.6319889440529972No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG39970.22854065044601735No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35370.20223874921880494No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG32990.18863037423602982No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC31540.18033955754484332No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT46250.084.332541
CGAGGGA13700.082.673133
ATAGCGG2800.082.336131
TAAGCGG3050.081.757651
AGGGATG57450.081.231995
AACGGGA10400.079.081083
CGCTACG7600.077.2972953
CGGGAAT6100.077.043865
ACGGGAT10800.077.022454
CGGGATA5250.076.985165
AAGGGAT62050.076.80054
AGCGGGA15800.076.444093
CGAAGGG14150.076.407962
GGATGGC33150.075.9887167
AAGGGAC25200.075.903494
TAGGGCG4300.075.41344
TAAGGGA30850.074.9510653
AGGGATT39200.074.931055
GCGGGAT12250.074.8111654
AGGGAAT32200.074.727765
Produced by FastQC (version 0.11.5)