FastQCFastQC Report
Mon 27 Feb 2023
SRR3127826.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127826.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1604532
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99320.6189966918702774No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG46170.2877474553327699No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG37110.23128239262289568No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC35550.22155993149404313No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32410.2019903622987887No Hit
AAAGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTT17600.10968930504346439No Hit
AGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCT17260.10757030710512472No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT41700.082.209461
AGGGATG50250.080.991865
CGAGGGA10050.080.898333
CGAAGGG12250.080.564332
TAGCGGG7250.079.73092
GCGGGAT12250.079.413414
TAAGCGG3300.078.3752751
AGGGAAT31250.078.351495
AGGGATT34500.077.6453255
AAGGGAT53650.077.523454
CGCTACG7200.077.020983
AGCGGGA17100.076.952283
AAGGGAC22300.076.4999854
AATGCGG3100.075.8470461
TAAGGGA25450.075.525763
ACGGGAT10000.075.193374
CGTAGGG3700.074.939342
AGAGGGA59500.074.8774263
GAAGGGA54150.074.81153
ATAGCGG3600.074.456511
Produced by FastQC (version 0.11.5)