FastQCFastQC Report
Mon 27 Feb 2023
SRR3127825.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127825.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1995692
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145700.7300725763294136No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC41720.2090502943339954No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG40680.2038390693553915No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG36670.18374578842827452No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC35700.1788853189770766No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT52300.083.915271
CGAAGGG16650.081.860562
GGGCGAT9150.081.157176
ATAGCGG3000.079.966841
AGGGATG68800.079.789425
GCGGGAT14900.078.5423664
AAGGGAT73850.078.406334
CGAGGGA16200.077.461723
AAGGGCG10800.077.4617164
AGGGATT45350.076.79485
AAGGGAC29200.076.776174
TAAGGGA34550.076.722363
TAGCGGG8350.075.986812
AATGCGG4400.075.904351
AGGGCGA12200.075.892235
AGCGGGA19100.075.789373
AGAGGGC33400.075.142523
TAGGGCG4450.074.9875644
GGATGGC41650.073.9123847
ACGGGTA2550.073.724324
Produced by FastQC (version 0.11.5)