FastQCFastQC Report
Mon 27 Feb 2023
SRR3127824.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127824.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10282417
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3987423.8779014700531986No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT457190.44463281347177414No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCT237880.23134638480427316No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT233920.22749515021614083No Hit
CGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT233580.22716448866059413No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG181650.1766607987207677No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT141610.13772053788520736No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT139560.13572684321205802No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTC114900.11174415509505208No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC112710.10961430566373645No Hit
CGTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC110960.10791237118665777No Hit
GAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGA106890.10395415785996619No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103200.10036550744829742No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1165150.090.655951
AGGGACT230400.070.39055
ATAGGGA66500.069.905133
GGTAAGG55500.069.885171
ATAGGGC46100.069.780373
GACTTAA181950.067.334958
AGTAGGG140000.067.285552
AGATAGG49850.067.217541
GTTTTTT1583800.066.9652252
AAGGGGC343100.066.207944
GTAGGGA64250.066.195453
TAGGGAC44750.065.886254
AGTAAGG59000.065.819311
AATAGGG125850.065.344782
TAGGGAT42850.065.2903754
GGACTTA191150.065.1267247
TAGTAGG32500.064.964011
GTATAGG29550.064.272581
GGATAGG53500.064.1291961
GATAGGG136200.063.975622
Produced by FastQC (version 0.11.5)