FastQCFastQC Report
Mon 27 Feb 2023
SRR3127823.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127823.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7099429
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2412233.3977803003593667No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT273320.3849887082468182No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCT237500.3345339463215985No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT237500.3345339463215985No Hit
CGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT223220.3144196526227673No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC200460.2823607363352743No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG174200.24537184610198934No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTC114580.16139326134538426No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC110600.15578717668702652No Hit
CGTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC106400.14987120795207615No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT82550.1162769569214651No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT81670.11503742061509453No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGC71510.10072641053245268No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT749950.090.492551
TAGTAGG23400.070.339771
ATAGGGC29150.070.296973
AGGGACT141600.069.932785
GGATAGG36150.069.8577651
ATAGGGA42750.069.261693
GACTTAA108100.068.951178
GGTAAGG38050.068.594151
AGATAGG35200.068.269541
GTTTTTT1010400.067.158712
AGTAGGG100200.066.88682
ACTTAAT111000.066.853389
TATAGGG44000.066.012242
GTAGGGA44650.065.9986043
ATAGAGG45450.065.7035141
GGACTTA115950.065.136537
AAGGGGC233600.064.9249654
AATAGGG84350.064.244042
GAATAGG38100.064.1840441
TAGGGAC30900.064.033794
Produced by FastQC (version 0.11.5)