FastQCFastQC Report
Mon 27 Feb 2023
SRR3127822.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127822.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10190229
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3718683.649260482762458No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT456930.4484001291825728No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCT236230.2318201092438649No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT235310.2309172836056972No Hit
CGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT234810.23042661749799734No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG185220.18176235293632753No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC141820.13917253478798172No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT141080.13844634894858596No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT139330.13672901757163652No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTC118770.11655282722301923No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC114880.11273544490511449No Hit
CGTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC114240.11210739228725872No Hit
GAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGA107450.10544414654469492No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1142350.090.433421
AGGGACT234650.070.419965
ATAGGGC48650.069.64983
ATAGGGA62800.068.100333
AGTAGGG145050.068.07382
TAGTAGG32700.068.0594251
GTTTTTT1556950.066.696212
GACTTAA185450.066.548058
GGATAGG53650.066.3897551
ATAGAGG62300.066.385881
GGTAAGG57500.065.8723141
AAGGGGC340650.065.766424
AGATAGG50350.065.5078661
GTAGGGA70000.064.989733
TAGGGAC45900.064.8124544
GAATAGG54600.064.631391
AGTAAGG57350.064.567831
AATAGGG124700.064.515192
GGACTTA194250.064.5010157
TATAGGG68800.064.03682
Produced by FastQC (version 0.11.5)