FastQCFastQC Report
Mon 27 Feb 2023
SRR3127821.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127821.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12396635
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4661993.7606898968954074No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT555740.4482990747085802No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCT253770.2047087778255954No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT246710.1990136839553637No Hit
CGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT231660.1868732926314278No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG208870.16848927148375345No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT172080.13881186305799922No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT168990.136319251151623No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132330.10674670989345093No Hit
GAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGA131020.10568997151243059No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTC129990.10485910087697187No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC128020.10326995995284204No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1377050.090.107491
AGGGACT276800.070.274435
ATAGGGA83600.069.7412643
AGATAGG61300.069.084811
TAGTAGG40400.069.067511
ATAGGGC54350.068.5172653
AGTAGGG180100.068.248972
GGTAAGG67900.068.1215061
AGTAAGG70400.067.440221
GACTTAA220400.067.165138
ATAGAGG83000.066.783271
GTTTTTT1872350.066.497042
AAGGGGC423300.065.6370244
GGATAGG67650.065.521471
GAATAGG67800.064.474291
GGACTTA232250.064.466627
TAGGGAC57400.064.386354
GTAGGGA82200.064.122173
TAGAGGG221800.064.00382
TATAGGG85400.063.871092
Produced by FastQC (version 0.11.5)