FastQCFastQC Report
Mon 27 Feb 2023
SRR3127820.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127820.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8305303
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3327124.006018805093565No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT320030.3853321185271627No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCT210420.2533561990453569No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTT193980.23356161719807214No Hit
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT187140.22532591526161055No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT113760.1369727269432554No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT109070.13132573248682197No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG104570.12590750752862356No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTC97770.11771996759179044No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96350.11601021660498118No Hit
CGTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTC92140.1109411661440889No Hit
GAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGA87870.10579987268375399No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT932500.090.5051041
ATAGGGC32550.071.042833
ATAGGGA53400.069.7629853
AGGGACT182450.069.2112355
GGTAAGG45950.068.4131241
AGTAGGG120350.068.326712
TAGTAGG27550.068.25751
TAGGGAT33000.068.218744
GTAGGGA51100.067.096563
GTTTTTT1267050.066.799142
ATAGAGG56500.066.649581
AGATAGG42450.066.282021
GACTTAA138950.066.155068
AGTAAGG48700.066.098391
TAGGGAC35300.065.775184
AAGGGGC289850.065.6444244
TAGAGGG151700.065.2588652
AATAGGG101750.065.033082
GATAGGG109650.064.685772
GGATAGG40400.064.5123441
Produced by FastQC (version 0.11.5)