FastQCFastQC Report
Mon 27 Feb 2023
SRR3127819.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127819.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5720796
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2015163.522516796613618No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCT215140.37606654738256706No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTT206400.3607889531456811No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT196990.3443401932178669No Hit
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT192740.3369111571186947No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC160340.2802756819155936No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG101910.17813954561568004No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTC99800.1744512476935028No Hit
CGTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTC93570.1635611547763633No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT68160.11914425894578307No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT65130.11384779320919676No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC59160.1034121825004772No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGC58240.10180401468606816No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT590650.090.723961
ATAGGGC24200.071.4693763
AGATAGG27000.070.725561
ATAGGGA35550.070.597483
GTATAGG15550.069.8708041
TAGTAGG17450.068.731991
GGTAAGG29600.068.644571
AGGGACT116800.068.442565
AGTAGGG79750.067.772692
GGATAGG28750.067.4021151
GACTTAA88050.067.145378
GTTTTTT802050.066.954592
TAGGGAT22200.066.896394
TATAGGG38100.066.7359542
ATAGAGG34700.066.552781
AATAGGG66200.066.238622
AGTAAGG30800.065.97011
AAGGGGC191400.065.829944
GAATAGG31150.065.530851
ACTTAAT90000.065.429469
Produced by FastQC (version 0.11.5)