FastQCFastQC Report
Mon 27 Feb 2023
SRR3127818.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127818.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8274473
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3099683.746075429818914No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT318850.385341761342384No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCT204860.24758072205927797No Hit
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT198430.23980983441483222No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTT194630.23521739692666832No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT116760.14110868450474126No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT107860.130352712492989No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC107460.12986929802055067No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG102560.12394747073318144No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTC98910.1195363136721819No Hit
CGTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTC95270.11513724197299333No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89950.10870782948956387No Hit
GAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGA87560.10581942801674499No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT899050.090.3722461
ATAGGGC35950.072.945893
ATAGGGA53750.071.434693
GAATAGG47750.069.978151
AGGGACT179950.069.939785
TAGTAGG27800.069.916991
GTATAGG24250.067.925651
AGTAGGG121700.067.456942
GGATAGG42200.067.3599851
ATAGAGG55200.067.183811
TAGGGAC39150.067.103574
GGTAAGG45150.067.0240551
GTTTTTT1225000.066.743882
GACTTAA142050.066.698328
TATAGGG56400.066.610182
AGATAGG43700.066.3401951
AATAGGG108250.066.0219962
AAGGGGC285900.065.604434
GTAGGGA55550.065.228283
TAGGGAT34250.065.040424
Produced by FastQC (version 0.11.5)