FastQCFastQC Report
Mon 27 Feb 2023
SRR3127816.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127816.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1261212
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106820.8469630799580087No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG45160.35806827083789244No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC40480.32096110725238897Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG40470.32088181844130886No Hit
AGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT16770.13296733618138742No Hit
GGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT13620.10799136069114472No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT39600.087.898951
ATAGCGG2700.083.622781
CGTAGGG3700.080.0310752
CGGGTAT1250.078.957735
TAGGGCG2450.078.653924
GTACGGG3050.078.594152
TAGGGCA10600.077.5951844
AGGGATT22900.077.373335
AAGGGCA22350.076.54674
TAGCGGG5650.076.534842
AATGCGG2100.076.156461
TAAGGGA13900.075.744843
AGGGATG25950.075.523845
AGGGCAT13950.075.467375
ATAGGGA17900.075.361363
GTAGGGC6750.075.2038043
GGCGATG3750.075.191877
ATTGCGG1950.074.778061
AAGGGAT36000.074.548044
AAGGGCG5500.074.3462754
Produced by FastQC (version 0.11.5)