FastQCFastQC Report
Mon 27 Feb 2023
SRR3127815.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127815.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1493875
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148290.9926533344490001No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG41670.27893900092042506No Hit
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG41240.27606058070454353No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC40960.27418626056396955Illumina Single End Adapter 1 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT15690.1050288678771651No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCCG156.904381E-493.993989
CGTTTTT54450.086.4766541
TAGGGCA12000.078.328314
GAGGGAT29350.076.2199864
TAAGGGA19500.076.159223
TAGCGGG5650.075.694272
GCGGGAT9750.075.677194
AGGGATG30550.075.379795
TAGGGTA5250.075.195184
AGAGGGA46600.075.134673
ATAGCGG2900.074.614371
ATAGGGA23600.074.478283
AAGGGAT43150.074.280294
GTAGGGC7750.073.982353
TTACGGG2100.073.852412
AGGGCAT18550.073.725745
AAGGGCA29100.072.998764
AGTAGGG22850.072.809342
AGAGGGC24400.072.61423
TAGAGGG26650.072.479412
Produced by FastQC (version 0.11.5)