FastQCFastQC Report
Mon 27 Feb 2023
SRR3127814.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127814.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1180668
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106640.9032175006013545No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG39850.3375207933136157No Hit
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG35240.2984751005363066No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC35010.29652705078819785Illumina Single End Adapter 1 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT14500.12281183194598312No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT38150.086.859431
ATAGCGG2450.086.577011
CGTAGGG3450.080.55872
AGGGATG24050.079.72485
TAGCGGG5650.079.205382
TAGGGCA10650.078.731754
TAAGGGA15350.078.254863
GAGGGAT23650.077.48154
GAATAGG6950.077.3172151
AGGGCAT15200.076.675475
AAGGGCA22150.076.561134
CGAGGGA8050.076.072393
CGAAGGG9050.075.474282
AGGGATC15400.075.374535
AAGGGAC14400.075.239324
AAGGGAT34600.075.01624
ATAGGGC10950.074.4237443
AAGGGCG5800.073.908264
ATAGGGA16200.073.8581163
GGGCGAT5050.073.516376
Produced by FastQC (version 0.11.5)