FastQCFastQC Report
Mon 27 Feb 2023
SRR3127813.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127813.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1780059
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162370.9121607766933567No Hit
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC48430.2720696336469746Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG47790.2684742472019186TruSeq Adapter, Index 23 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG45750.2570139529083025TruSeq Adapter, Index 23 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT55300.084.474741
ATAGCGG3900.079.612641
TAGCGGG9000.076.2405242
TAGACGG2300.075.6792141
CGTAGGG4750.075.196142
AGTACGG1600.073.505991
CGAAGGG15250.072.730692
GTACGGG3850.072.022272
ATAACGG1200.070.565761
ACGGGAT5750.070.290064
TAGGGCA11300.070.2864464
AGGGCAT17400.069.684125
TAGGGTA7100.069.501524
GTATAGG7250.068.781331
ATAGGGC11300.068.6247943
GAGGGAT30450.068.372234
AAGGGCG6050.068.35824
GAATAGG11300.068.2761
TAGAGGG30500.068.262072
AGGGATT27600.067.769975
Produced by FastQC (version 0.11.5)