FastQCFastQC Report
Mon 27 Feb 2023
SRR3127812.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127812.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2131207
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220811.0360795549188793No Hit
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC47240.2216584311143873Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG47180.22137690050755277TruSeq Adapter, Index 23 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG44770.21006875446636578TruSeq Adapter, Index 23 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74950.084.759721
ATAGCGG4550.079.6355061
CGTAGGG5550.078.754792
TAGCGGG10000.075.6681062
TAGACGG3550.074.23131
TAGGGCA12650.070.9626544
CGAAGGG19850.070.79422
AGGGATG32850.070.677085
AGATAGG13400.070.586011
CGAGGGA12950.070.044583
ACGGGAT7550.069.720114
GACCGAT1350.069.627898
AAGGGAT45850.069.498814
TAGAGGG36650.068.9914552
AGGGCAT21500.068.640155
AGAGGGC31450.067.9951253
AGGGATT35900.067.945375
TAACGGG5400.067.887192
AAGAGGG75750.067.8768542
TAGGGCG4300.067.765754
Produced by FastQC (version 0.11.5)