FastQCFastQC Report
Mon 27 Feb 2023
SRR3127811.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127811.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1660935
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160560.9666844277470219No Hit
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG42790.25762597573053736TruSeq Adapter, Index 23 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG42180.2539533455553649TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC41580.2503409224322445Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT55250.084.703461
CGTAGGG4250.082.01212
ATAGCGG3300.081.4038851
CGGGTAT1650.076.901235
TAGCGGG8250.073.078762
TAGAGGG28600.071.969842
AGAGGGC24750.071.556283
TAGGGCA10200.071.114094
CGAAGGG15200.070.96222
AAGGGAT35850.070.553784
CGAGGGA9500.070.404423
ACGGGAT5850.070.0484544
AGGGATG25050.069.976885
AGGGCAT16250.069.6974955
AAGAGGG56450.069.588462
TAAGGGA16400.069.50353
TAGACGG2650.069.359021
GAGGGAT29150.069.157674
AGGGAAT20600.068.895885
ATAGGGA17450.068.560423
Produced by FastQC (version 0.11.5)