FastQCFastQC Report
Mon 27 Feb 2023
SRR3127809.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127809.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971695
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129611.3338547589521403No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG34600.35607881073793735No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG34480.35484385532497337No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC33230.3419797364399323No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA17220.177216101760326No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC11450.11783532898697636No Hit
AGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT10560.1086760763408271No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG2550.088.463952
CGTTTTT40450.087.8894651
TAGACGG1050.085.093791
TAGGGCG1250.082.71384
ATAGCGG1600.082.294651
AGTACGG1100.081.225891
AGGGTAC3200.080.775195
TTGACGG1800.078.3758541
GGTAAGG4350.077.8353351
TACGGGT1100.076.903333
TAGGGCA5500.076.048844
TAGCGGG4350.075.626512
GAATAGG5500.075.240821
GCATAGG2200.074.813321
TAACGGG2400.074.411092
TAAGAGG7050.074.040171
ACGGGTA1150.073.55974
ATAGGGC6250.072.938533
TAGAGGG16200.072.815522
AAGGGCG3750.072.687884
Produced by FastQC (version 0.11.5)