FastQCFastQC Report
Mon 27 Feb 2023
SRR3127807.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127807.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1989487
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145980.7337569936370532No Hit
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG48540.2439824939796038TruSeq Adapter, Index 27 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG45230.22734503919854715TruSeq Adapter, Index 27 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC44420.22327363787750307TruSeq Adapter, Index 27 (100% over 24bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC22570.11344633063699336No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62750.085.2402341
CGTAGGG5900.083.636982
AGGGATG45550.079.337255
TAGCGGG9100.079.015282
GGGCGAT7850.078.420566
TAGGGCG3400.077.4013064
AGGGATC26650.076.8826755
CGAGGGA14700.076.726263
CGAAGGG18950.076.632032
ATAGGGA29250.075.995063
TAGGGAC13000.075.551344
ATAGCGG4050.075.501621
AAGGGAT57100.074.8112644
AGTAAGG12500.073.763911
TAGGGAT26300.072.90274
AAGGGCG8000.072.8401644
GAGGGAT50400.072.262064
GCGGGAT8550.072.0019764
TCGAAGG2550.071.94861
TAACGGG3600.071.799472
Produced by FastQC (version 0.11.5)