FastQCFastQC Report
Mon 27 Feb 2023
SRR3127805.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127805.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868975
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141460.7568854586069904No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG41380.2214047806953009TruSeq Adapter, Index 27 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG40820.21840848593480383TruSeq Adapter, Index 27 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC37920.20289195949651545TruSeq Adapter, Index 27 (100% over 24bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC19170.10256959028344415No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG3450.084.681441
CGTTTTT60300.083.692751
CGTAGGG4650.080.023052
AGGGATC27100.079.9436345
AGGGATG44350.079.473065
TAGCGGG9550.079.407852
AAGGGAT58450.078.087274
CGAGGGA13250.076.785423
CGAAGGG16700.076.153212
TAGGGAT24950.075.136944
GAGGGAT46550.074.9789664
TAGGGCA13650.074.880394
ATAGGGA28750.074.871983
CGGGATG7400.074.303015
TAAGGGA27850.073.7398453
AGGGACT22050.073.316575
GCGGGAT10000.073.008374
ACGGGAT7900.072.740074
AAGGGCA29600.072.721944
GGATGGC27800.072.183077
Produced by FastQC (version 0.11.5)