FastQCFastQC Report
Mon 27 Feb 2023
SRR3127804.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127804.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2452158
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237450.968330751933603No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG40650.16577235235249932TruSeq Adapter, Index 23 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG37470.15280418309097538TruSeq Adapter, Index 23 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC34350.14008069626834813TruSeq Adapter, Index 20 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31770.12955935139579097No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT72500.084.0714341
GAATAGG16800.073.345491
TAGGGCA15850.071.757344
CGTAGGG5250.071.620532
ATAGGGC14850.071.5271763
ACGGGTA2800.070.497144
AGATAGG15250.070.0063551
AAGGGCG12350.069.2603454
TAGGGTA5050.068.868494
TAGTAGG9600.068.586431
GATAGGG36300.068.494522
TAGACGG3250.068.0136261
TAGGGTC7750.067.919834
ATAGAGG19350.067.81171
ATAGGGA17100.067.612673
TAGAGGG50450.067.170852
AAGAGGG86650.066.8807752
AGTAGGG38100.066.738912
ATAAGGG36550.066.2257162
AGGGCAT24800.065.759435
Produced by FastQC (version 0.11.5)