FastQCFastQC Report
Mon 27 Feb 2023
SRR3127803.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127803.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2935735
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323201.1009168061831194No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38750.13199420247399715No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG38170.13001854731438633TruSeq Adapter, Index 23 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG37060.1262375520951312TruSeq Adapter, Index 23 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC32910.11210139879791602TruSeq Adapter, Index 20 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT96850.085.337971
CGTAGGG7950.076.854442
TAGGGCA22100.071.88154
TAGGGTC9100.071.790364
TAGGGTA8650.070.6354
ATAGGGC19000.070.004443
GGATAGG20000.069.384451
TAGAGGG59600.068.8431552
AGGGCAT30200.068.787285
ATAGGGA23650.067.567913
AAGGGTA23650.067.369194
AGTAGGG46250.067.272732
GAATAGG18500.066.873511
AGGGATG35000.066.87355
ATAGAGG22450.066.841251
AGATAGG18300.066.833221
AGGGTAT15600.066.582555
AAGAGGG104800.065.790262
ATAAGGG42200.065.487382
ATAACGG3450.065.447391
Produced by FastQC (version 0.11.5)