FastQCFastQC Report
Mon 27 Feb 2023
SRR3127802.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127802.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2268841
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236441.0421179800611853No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG36210.15959690432251533TruSeq Adapter, Index 23 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG33660.14835768570825367TruSeq Adapter, Index 23 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30920.13628103511881176No Hit
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC29190.12865599660795973TruSeq Adapter, Index 20 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70000.086.7881
TAGGGCA15150.072.763924
CGTAGGG6350.072.705162
ATAGGGA16600.070.948753
TAGAGGG44900.070.5075762
ATAGGGC14650.069.780643
AGTAAGG13400.068.585871
AAGGGTA18150.068.2640464
ATAGAGG17500.067.8683551
AGTAGGG36050.067.822682
GTATAGG7450.067.691211
AGGGATG26900.067.609525
AGGGCAT23400.067.4796145
GAATAGG14500.067.283281
AAGAGGG82200.067.111662
TAGGGTA5550.067.057444
GAGGGAT25600.066.616454
AGAGGGC37450.066.1678163
AAGGGCA38450.066.1622544
AGGGTAA9950.066.1232455
Produced by FastQC (version 0.11.5)