FastQCFastQC Report
Mon 27 Feb 2023
SRR3127801.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127801.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2643453
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT488411.8476212741440834No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG32840.1242314503038261No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT32170.12169688661005133No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT30660.1159846609718425No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC27350.10346315973841788No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT148700.087.970871
TAGACGG3850.074.527741
AGGGATG41600.071.286445
AGTAGGG47250.070.9172
ATAGGGC14600.070.494283
CGTAGGG8150.070.350122
GAGGGAT37050.070.145464
ACGGGAT10100.069.331014
AAGAGGG99550.069.160642
AGAGGGC41300.067.3653
AAGGGAT49700.067.326544
GAATAGG14950.066.702721
GTAAGGG41250.066.535212
ATAGGGA26700.066.5339363
ATAAGGG35750.066.123312
AGATAGG18300.066.058841
TAGGGCA16450.065.708964
TAGAGGG49000.065.21922
GTAGGGA19600.065.219193
GGTAAGG17100.065.193021
Produced by FastQC (version 0.11.5)