FastQCFastQC Report
Mon 27 Feb 2023
SRR3127800.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127800.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3138968
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630022.007092776989125No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG32150.10242219735913205No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT199050.087.278891
TAGACGG4600.073.632031
AGTAGGG52200.073.466532
TAGGGCA22050.070.975024
GAGGGAT43300.070.224124
AGGGATG50150.069.628625
ATAGGGC21350.068.899543
AAGAGGG119650.067.991452
TAGAGGG58350.067.897942
AGGGTAC15350.067.357235
ATAGGGA33450.066.737113
AAGGGCA54250.066.532124
ATAAGGG44150.066.317362
GAATAGG20650.066.2925951
CGTAGGG11050.065.923362
TAGGGTA11350.065.837174
AGAGGGC50150.065.786393
AGAGGGA74450.065.7768253
AGGGCAT32850.065.237815
GTAGGGA23700.065.042223
Produced by FastQC (version 0.11.5)