FastQCFastQC Report
Mon 27 Feb 2023
SRR3127799.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127799.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2408110
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT472311.9613306701105848No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT30920.1283994501912288No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG29860.12399765791429793No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT26780.11120754450585728No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT142650.088.080341
AGTAGGG40050.073.772152
GAGGGAT32350.072.249494
ATAGGGC15500.071.443643
AGGGATG40550.071.157725
CGTAGGG7050.070.850692
AGAGGGC36400.070.0363163
AAGAGGG92350.069.59932
AAGGGCA41450.067.8698964
TAGAGGG42900.067.553092
ATAAGGG35100.067.528682
GTAGGGT7750.067.491443
AGAGGGA59700.067.407923
TAGGGCA16600.067.277174
AGGGCAT25750.067.160685
TAGACGG3100.066.9182361
AGGGTAC10450.066.106715
TATAGGG19650.065.7075652
AGATAGG16300.065.369381
ATGCGGG12150.065.15692
Produced by FastQC (version 0.11.5)