FastQCFastQC Report
Mon 27 Feb 2023
SRR3127798.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127798.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3352504
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296110.8832502511555542No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG42450.12662177285992798TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG41950.1251303503291868TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC37510.11188651825620492TruSeq Adapter, Index 21 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT104300.082.7040561
ATAGGGC21150.075.551543
TAGGGCA19200.075.1448064
CGTAGGG9500.073.7118762
TAGGGCG7200.068.535984
TAGCGGG17800.068.119972
GGTAAGG21950.067.926071
AGTAGGG54400.067.64532
AGGGATG40300.067.637065
GAATAGG17600.067.344111
ATAGCGG8000.066.43551
AAGAGGG119850.066.388632
CGAAGGG35500.066.326062
AGTAAGG19350.065.871881
AATAGGG49050.065.633622
GTACGGG8350.065.289942
TAGGGAT21100.065.259984
TATAGGG28250.065.214232
GGATAGG20250.065.034641
AAGGGCA56950.064.449444