FastQCFastQC Report
Mon 27 Feb 2023
SRR3127797.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127797.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4044298
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT399110.9868461720674392No Hit
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG43040.10642143580913176TruSeq Adapter, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT134100.084.1063461
CGTAGGG11650.078.259812
ATAGGGC28300.074.895053
TAGGGCA24650.071.6859364
AGTAGGG65500.070.458472
TAGGGCG8800.069.96034
AGGGTAC19700.068.7051545
GAATAGG23000.067.920331
AGGGATG51200.067.189925
CGAAGGG39250.067.051942
GGTAAGG25350.066.821171
ATAGGGA35200.066.7560043
TAGAGGG76600.066.506412
AGAGGGC63850.065.654883
AAGAGGG143250.065.647092
AAGGGCG16700.065.569594
TAGCGGG20550.065.4059452
GTAGGGT16450.064.851943
AGGGCAT42600.064.537075
AAGGGCA73500.064.515914
Produced by FastQC (version 0.11.5)