FastQCFastQC Report
Mon 27 Feb 2023
SRR3127796.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127796.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3115141
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288380.9257365878462644No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG36200.11620661793479012TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG36040.1156929975240286TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC31750.10192155026048581TruSeq Adapter, Index 21 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT99050.083.357691
ATAGGGC22050.076.9067153
TAGGGCA20100.074.056194
ATAGCGG6500.071.777431
CGTAGGG8800.071.728622
AGTAGGG50700.070.239952
TAGCGGG16000.069.774822
GGTAAGG18100.069.5182341
CGAAGGG32150.069.449282
AGGGTAC14900.068.442355
GAATAGG17200.067.9499741
ATAGGGA25000.067.454893
AAGAGGG111550.067.438162
AGAGGGC50000.067.360673
AGGGCAT31450.067.092995
TAAGGGA27500.066.632683
TAGGGTA10900.065.688224
AAGGGTA23800.065.5120854
AGGGATG37500.065.291695
TAGAGGG58000.064.9729162
Produced by FastQC (version 0.11.5)