FastQCFastQC Report
Mon 27 Feb 2023
SRR3127795.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127795.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2229894
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217620.9759208285236876No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC43790.19637704751885066No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG41820.1875425468654564TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG40600.18207143478568938TruSeq Adapter, Index 14 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22830.10238154818121399No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT65300.084.789211
TAGGGCA14250.075.193164
CGTAGGG4950.075.003282
ATAGCGG4350.071.372681
ATAGGGC12700.071.048663
AAGGGTA15700.070.94264
ATAAGGG30000.070.493592
AGGGCAT22650.070.3395545
AGTAGGG33900.070.0083852
ATAGGGA19400.069.2823643
AGGGTAC10050.068.273935
AGAGGGC34250.068.0580443
GGTAAGG10550.067.328951
TAGAGGG38950.066.9643862
GTAGGGT7950.066.7989653
AGTAAGG13650.066.512311
GAATAGG13600.066.410941
AGGGTAT13600.066.3484045
ATAGAGG15850.066.183991
TAGTAGG7550.066.044431
Produced by FastQC (version 0.11.5)