FastQCFastQC Report
Mon 27 Feb 2023
SRR3127794.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127794.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2724497
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298561.0958353046452243No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG39960.14666927509922015TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG39460.14483407395934003TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC38020.13954869467648523No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27820.10211059142293054No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT90550.084.076231
CGTAGGG5450.075.024562
TAGGGCA15450.073.006834
AGGGCAT27250.072.437515
ATAGCGG5400.071.4501651
ATAGGGA21900.070.604523
AGTAGGG43850.070.202482
ATAAGGG41600.070.045322
ATAGGGC14800.069.8621143
TAGAGGG48600.069.82032
TAGGGTA10700.068.95994
GTAGGGT11450.068.5475163
GGTAAGG13250.068.181811
AGAGGGC43400.067.681663
AAGGGTA20550.066.5521244
ATAACGG3850.065.995781
TATAGGG20950.065.954442
AGGGTAT17550.065.3421555
ATAGAGG20400.065.273881
AGTAAGG16800.064.977331
Produced by FastQC (version 0.11.5)