FastQCFastQC Report
Mon 27 Feb 2023
SRR3127793.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127793.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2006936
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213061.0616183077088657No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC38990.19427624996512094No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG37870.18869560364655374TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG36460.18166996854907183TruSeq Adapter, Index 14 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT20750.10339143849131213No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64150.085.071461
CGTAGGG5750.081.9358442
ACGGGTA1600.073.6142354
TAGGGCA10400.072.028694
ATAAGGG27150.071.840992
ATAGGGC9800.070.669663
AGGGTAT12500.070.3038565
AGGGCAT20000.070.256865
AGAGGGC32200.069.206523
TAGCGGG8850.069.2056962
ATAGAGG12950.069.14421
GGTAAGG11350.068.926181
TAGAGGG32550.068.896582
TAGGGAT11600.067.826644
AGTAGGG32750.067.756562
GAATAGG12600.067.3246151
AAGGGTA14650.067.212564
ATAGGGA16500.067.100493
AAGGGAT28950.066.885974
GAGGGTA9900.066.6245964
Produced by FastQC (version 0.11.5)