FastQCFastQC Report
Mon 27 Feb 2023
SRR3127792.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127792.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530730
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58511.1024438038173836No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG34270.6457143933826993No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC33420.6296987168616811No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG33010.621973508186837No Hit
AGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT10910.20556591864036325No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA9890.1863471068151414No Hit
ACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG9110.17165036836055997No Hit
GGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT8760.165055678028376No Hit
CGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT8550.16109886382906563No Hit
AGAGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT8010.15092419874512464No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGCTTAACA7590.14301057034650386No Hit
GGAGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT7060.13302432498633956No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT6790.12793699244436907No Hit
AAAGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT6650.12529911631149548No Hit
GCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT6350.11964652459819493No Hit
ACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT6090.11474761178000112No Hit
TCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG5580.10513820586739019No Hit
AGGCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT5410.10193507056318654No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTAGG1350.094.0612951
TACCGGG201.8370543E-593.999242
CGATTAC156.9055386E-493.981549
CGTTTTT20950.086.653121
TAGGGAG2800.083.912094
TACGGGT401.035005E-982.2415853
CGTAGGG1500.081.466012
TAGCGGG2400.080.291022
AGGGCGA1300.079.5228355
TCATAGG600.078.3844151
AGGGAAT8000.076.947385
GGGCGAT1350.076.5775456
AGAGGGT2850.075.85193
AGTAGGG7050.075.3327262
CGAGGGA2250.075.1923143
AGGGTCC1750.075.185235
AGGGTAC1450.074.537085
ACGGGTG900.073.096754
AAGGGAC2700.073.096754
ATAGAGG2850.072.608721
Produced by FastQC (version 0.11.5)