FastQCFastQC Report
Mon 27 Feb 2023
SRR3127791.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127791.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613234
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87291.4234370566537407No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG36220.5906391361209586No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC33830.5516654327711771No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG32330.5272049494972555No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA12240.19959754351519973No Hit
AGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT10970.17888766767661285No Hit
ACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG9130.14888280819393576No Hit
CGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT8380.136652566556975No Hit
GGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT7720.12588995391644953No Hit
AGAGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT7590.12377004536604297No Hit
AAAGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT7170.11692111004934495No Hit
ACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT6990.11398585205647435No Hit
GCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT6720.1095829650671685No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT6540.10664770707429791No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCGCTTAACATCGT6280.10240788997348484No Hit
TCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG6270.10224482008499203No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGTA201.8366087E-594.006294
GTACGGG1100.093.9985352
AGTACGG750.087.79641
CGTTTTT27050.085.5475851
AGGGTAC2350.079.998755
CGTAGGG1450.077.7918852
GAATAGG2850.077.564481
AGGGAAT10650.076.787545
TAGGGAG3200.076.37384
CGAAGGG4250.076.3046952
AGTAGGG7850.076.0370252
TTAACGG507.094059E-1175.254051
TAGCGGG2250.075.198832
TAGGGCG1000.075.198824
CGAGGGA3700.074.944773
GCAAGGG8000.074.6113362
TAGGGAC1650.074.059454
CTATAGG700.073.910231
TAGAGGG8150.073.238122
GCGTAGG900.073.1636661
Produced by FastQC (version 0.11.5)