FastQCFastQC Report
Mon 27 Feb 2023
SRR3127790.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127790.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491321
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58891.1986053924013018No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG29170.5937055407768037No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG28600.5821041640801025No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC28100.5719275178549258No Hit
AGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT9560.1945774758253769No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA9420.19172801488232744No Hit
GGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT8350.1699499919604495No Hit
CGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT8250.16791466271541416No Hit
ACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG7270.14796843611406799No Hit
AGAGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT6680.13595999356835958No Hit
GGAGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT6000.12211975470211937No Hit
AAAGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT5810.11825262913655228No Hit
GCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT5720.11642083281602049No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT5630.1145890364954887No Hit
ACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT5570.1133678389484675No Hit
TCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG5250.10685478536435447No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATAGG353.5106495E-1094.230181
ACGGGTA550.094.210954
CGTATTG156.9026323E-493.990439
TCGTCTC550.093.990435
CGTTTTT19950.085.728211
GATTAGG1250.082.9225541
TATCGGG353.8004146E-880.752252
TAGGGCG900.078.5091254
TAGCAGG1350.076.780141
CGAGGGA2150.076.6833343
AGGGAAT9250.076.208465
TAGCGGG1550.075.976582
TGCAAGG1800.075.9076461
TAGTAGG1500.075.384151
TAGGGAC750.075.368774
CGTAGGG1000.075.368762
AGGGCGT750.075.192355
CGAAGGG3700.075.114142
ATGTAGG1450.074.734281
GCATAGG1200.074.598891
Produced by FastQC (version 0.11.5)