FastQCFastQC Report
Mon 27 Feb 2023
SRR3127789.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127789.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3562907
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT401101.1257661230001232No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG44230.124140203491138No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC40290.11308181774040131No Hit
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG36300.10188309714511212No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT137150.085.2740551
CGTAGGG10500.073.8538742
GAGGGAT52650.071.501114
TAGGGAT28700.071.233794
GAATAGG23800.070.962531
ATAGGGA36800.070.6245963
TAGACGG4250.069.7371
AGGGATG57050.069.693675
AGTAGGG61150.069.632252
TAGAGGG65050.069.358962
GGTAAGG21900.068.956071
ATAGGGC22750.068.792573
TAGCGGG20350.068.591562
ATAGCGG9100.068.240811
AAGGGAT73550.067.988844
GTAGGGA29450.067.983413
TAAGGGA37500.067.802343
AGATAGG23000.067.7036061
TAGTAGG16200.067.0824051
TAGGGTA9850.066.321944
Produced by FastQC (version 0.11.5)