FastQCFastQC Report
Mon 27 Feb 2023
SRR3127788.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127788.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4294825
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540251.2579092279662152No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG43020.10016706152171509No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT182850.085.470531
CGTAGGG13300.079.5046162
ATAGGGA46650.073.138573
ATAGGGC28250.072.365623
TAGGGCA27200.071.185224
TAGAGGG81050.070.9724662
AGTAGGG71700.069.281512
AGGGATG71050.068.592435
GAGGGAT63200.068.0401844
TAGGGAT37950.067.9864044
AAGGGAT95300.067.609244
GTAGGGA36200.067.3783343
GAATAGG26950.067.206871
TAGACGG4800.066.646811
AATAGGG66650.066.5630652
ATAGCGG10050.066.4712751
GGTAAGG25300.066.3833851
AGAGGGC72600.065.833333
AAGAGGG166950.065.75792
ACGGGAT15600.065.673994
Produced by FastQC (version 0.11.5)