FastQCFastQC Report
Mon 27 Feb 2023
SRR3127787.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127787.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3338046
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT393561.179013111263296No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG38100.11413863080376964No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT132200.085.217361
CGTAGGG8400.073.489142
GGTAAGG19800.072.371911
AGGGATG55200.072.364485
AAGGGAT74600.072.137074
ATAGGGA32900.071.4719853
GAGGGAT52800.070.773474
AGGGATC36900.069.791065
TAGGGAT27500.069.227694
TAGAGGG61150.069.200792
TAAGGGA38850.068.773833
ATAGGGC18950.068.3840263
ATAGCGG8500.067.655411
TAGGGCA18800.066.9244164
AGTAGGG54850.066.753742
AGAGGGC51950.066.4889763
GTAGGGA27050.066.372613
AGAGGGA88950.066.0090263
AAGGGCA55900.066.005664
TATAGGG26300.065.93612
Produced by FastQC (version 0.11.5)