FastQCFastQC Report
Mon 27 Feb 2023
SRR3127786.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127786.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2722197
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266580.9792825427402939No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG35410.13007875623990475No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC32490.11935212624214926No Hit
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG29250.10744997514874934No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT90150.083.666231
AGGGATG51750.074.925865
ATAGGGA28750.073.2352753
ACGGGTA3600.073.106784
TAGGGCG5650.072.367394
CGAGGGA17600.071.2982253
ATAGGGC22300.071.024013
CGTAGGG8150.070.929622
TATAGGG23750.070.645532
AGTAGGG47250.070.223592
AAGGGAT58050.069.8683854
GAGGGAT46300.069.328514
CGAAGGG25250.069.05462
AGGGCAT31900.068.951435
TAGGGAT25050.068.666594
ATAGCGG5900.068.542181
AAGGGCA50600.068.452474
GATAGGG43650.068.370632
TAGAGGG53300.068.0722662
AAGAGGG97450.067.9530942
Produced by FastQC (version 0.11.5)