FastQCFastQC Report
Mon 27 Feb 2023
SRR3127785.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127785.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3299871
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360301.0918608636519427No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG34000.10303433073595906No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT118900.084.729371
ATAGCGG7950.075.168471
AGGGATG61650.074.552975
CGTAGGG8750.074.120252
TAAGGGA36850.072.82233
ATAGGGC24950.071.954613
TAGCGGG18050.071.861732
TAGGGCA23700.071.387144
CGAAGGG30750.070.762262
AAGGGCG15750.070.718594
GAGGGAT56200.069.742184
AGTAGGG57250.069.2839662
AGGGCAT41250.068.813275
TAGAGGG65500.068.593412
AAGGGCA64000.068.3652654
ATAAGGG51500.068.259042
ATAGGGA39600.068.239913
ACGGGAT13300.067.844624
AAGAGGG124150.067.5705262
TAGGGAT35350.067.004954
Produced by FastQC (version 0.11.5)