FastQCFastQC Report
Mon 27 Feb 2023
SRR3127784.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127784.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2534282
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262971.0376509007284904No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG33480.1321084236087381No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC29070.11470704523016775No Hit
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG27320.1078017363497827No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT88900.086.268361
ACGGGTA2750.080.536624
ATAGCGG5450.079.57141
TAGGGCA17800.076.77264
CGAAGGG22900.072.227042
ATAGGGC18250.071.52293
AGGGATG50650.071.349525
ATAGGGA29450.070.723733
AAGGGCG12950.070.5927664
AGTAGGG45800.070.580852
GAGGGAT45400.069.749594
AGAGGGC44350.069.48843
AAGCGGG30400.069.443672
CGAGGGA17050.068.265433
TAGGGCG5600.068.159314
AAGGGCA47850.067.753974
AAGAGGG92950.067.578572
TAGCGGG13950.067.559132
TAGAGGG53300.067.368362
GTAGGGC15750.067.317843
Produced by FastQC (version 0.11.5)