FastQCFastQC Report
Mon 27 Feb 2023
SRR3127783.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127783.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2452638
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259481.0579628954619476No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG28960.1180769440904039No Hit
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC27140.11065636265930806Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG26890.10963705202316851No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT78450.084.592271
CGTAGGG6500.073.750422
TAGGGCA17750.072.0191964
ATAGGGC17300.071.174433
GTAGGGT9050.071.144423
ATAGCGG5950.071.141351
TAGGGTA9000.070.496734
AGTAGGG42200.069.49442
TAGCGGG13850.069.224222
GTAGGGA18300.068.569193
TAAGGGA21300.068.3991853
GAATAGG16100.067.773311
AGGGATG37100.067.6478355
TAGAGGG50800.067.628752
AGTAAGG15100.067.5895161
ATAGGGA21250.067.4543153
ATAAGGG33450.067.159812
AAGGGCA46550.066.937814
AGAGGGC42000.066.914573
AAGAGGG90100.066.819322
Produced by FastQC (version 0.11.5)