FastQCFastQC Report
Mon 27 Feb 2023
SRR3127782.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127782.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2971150
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT345561.1630513437557848No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG29790.10026420746175724No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT102150.085.4386751
ATAGGGC22550.074.192913
ATAGCGG6250.073.772581
CGTAGGG8800.073.6979452
TAGGGTC8600.072.133084
TAGGGCA22750.071.6814964
GTACGGG7750.070.3420942
AGTAGGG49650.070.138732
TAGAGGG61650.070.055372
AGAGGGC50400.069.0953
AAGGGCA54600.068.686164
GGTAAGG17500.067.481481
GTAGGGA23050.067.282483
TAGCGGG17050.066.979352
AAGAGGG111700.066.812282
ATAGGGA28850.066.787783
TAGGGTA9250.066.5562064
AGGGATG44450.066.396755
AGATAGG19500.066.350931
AGGGCTC26500.066.1487965
Produced by FastQC (version 0.11.5)