FastQCFastQC Report
Mon 27 Feb 2023
SRR3127781.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127781.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2273216
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254651.1202191080829977No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG26210.11529920605872913No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG23010.1012222331709789No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75000.085.792991
CGTAGGG7300.078.7194752
GTAGGGT8200.072.951643
ATAGGGC16250.072.7554553
TAGCGGG12000.072.616562
ATAGCGG5850.072.521551
GTAGGGA15850.071.9169543
AGTAGGG39500.071.9060442
TAGGGCA16750.071.708494
TAGGGTC6250.071.596014
TACGGGT2000.070.6539463
ATAGGGA22850.070.293213
TAGAGGG43700.069.953342
GCATAGG6650.069.468021
AGTAAGG13500.069.1372151
AGGGCAA29100.068.958135
TAGGGTA8750.068.904434
ATAGGGT11100.068.320043
TGTAGGG17600.067.977662
ATAAGGG30350.067.821592
Produced by FastQC (version 0.11.5)