FastQCFastQC Report
Mon 27 Feb 2023
SRR3127780.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127780.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3277220
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT445991.3608790377209952No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG44460.13566376379980594RNA PCR Primer, Index 38 (95% over 22bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC40080.12229877762249712No Hit
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC39650.12098668993842342RNA PCR Primer, Index 38 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG38480.11741659089106012RNA PCR Primer, Index 27 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT140600.086.818721
CGTAGGG8800.074.7713852
TAGGGCA18600.073.782514
AGGGATG54750.071.763835
ATAGCGG8000.071.7350161
AGTAGGG54100.070.629052
GTACGGG7800.070.498742
AGTAAGG23200.069.950761
ATAGGGC19100.069.88253
GAGGGAT47450.069.333854
AGATAGG21100.069.3328861
ATAGGGA33100.068.8647843
ACGGGAT11400.068.8485954
TAGCGGG17550.068.289382
GAATAGG18750.068.238431
AAGAGGG118950.067.644022
AAGGGAT59400.066.8580554
ATGCGGG16250.066.811112
AGGGTAC11900.066.745695
AAGGGTA23250.066.707474
Produced by FastQC (version 0.11.5)