FastQCFastQC Report
Mon 27 Feb 2023
SRR3127779.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127779.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3937843
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT597671.5177598497451523No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC51390.13050291746014253No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG43410.11023801609155062RNA PCR Primer, Index 38 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC40310.10236568598595729RNA PCR Primer, Index 38 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT192900.086.730021
TAGGGCA23650.071.935434
AGGGATG65400.071.572635
TAGACGG5850.070.772851
AGTAGGG67600.069.521432
ATAGGGC22800.069.464113
CGTAGGG12400.069.357722
ACGGGAT15150.068.555944
ATAGGGA46050.068.0709153
TAGGGTA13050.068.063884
TAGCGGG18750.067.925592
AAGGGAT72900.067.5614854
AGATAGG26500.067.464861
AAGAGGG145200.067.322792
GAGGGAT58500.066.9197854
GAATAGG22900.066.565541
AGTAAGG26450.066.525151
GATAGGG67500.066.2824552
ATAGCGG8450.066.256751
AGAGGGC60300.066.169193
Produced by FastQC (version 0.11.5)