FastQCFastQC Report
Mon 27 Feb 2023
SRR3127778.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127778.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3067137
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT439751.4337474980739366No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG38730.12627411165526678RNA PCR Primer, Index 38 (95% over 22bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37160.12115533150296189No Hit
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC35090.11440636658877645RNA PCR Primer, Index 38 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG34310.1118632783602428RNA PCR Primer, Index 27 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT140950.087.691111
TAGGGCA18650.075.014264
ATAGGGA31550.073.7553943
AGTAGGG48250.072.536682
ATAGGGC18450.071.742443
CGTAGGG8700.070.9280852
AGATAGG19600.070.9231951
CGAGGGA20750.070.3736043
AGGGATG52250.070.245315
ACGGGAT10850.069.897614
GGTAAGG18050.068.659541
TAGAGGG53450.068.56432
AGAGGGC44550.067.9875643
GAGGGAT43200.067.713324
AAGAGGG111450.067.413492
CGAAGGG28000.066.956272
TAGGGCG4100.066.6362154
GATAGGG48500.066.626742
ATAGCGG7500.066.5988851
GTAAGGG49850.066.050822
Produced by FastQC (version 0.11.5)