FastQCFastQC Report
Mon 27 Feb 2023
SRR3127776.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127776.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966509
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144330.7339401955444903No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG31130.1583008264899881No Hit
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG28110.14294366311061887No Hit
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC25000.1271288359219307No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT63350.085.992161
CGAGGGA15900.080.099013
AAGGGAT65900.077.660014
CGAAGGG20500.077.485032
CGTAGGG4500.077.281262
TAGCGGG7250.077.137212
AGGGATC32700.076.8884355
TAAGGGA31400.076.479713
GAGGGAT53250.076.428134
AGGGATT49200.075.364515
AGGGATG52000.074.831075
TAGGGCA15800.074.657194
AGGGAAT33950.074.3343355
TAGGGAT30900.073.915034
GCGGGAT12800.073.79744
GACCGAT1600.073.430258
ATAGGGA33150.073.0093843
AGGGATA44550.072.681945
CGGGATT9700.072.673245
GGGCGAT10100.072.12166
Produced by FastQC (version 0.11.5)