FastQCFastQC Report
Mon 27 Feb 2023
SRR3127775.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127775.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1526203
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106860.7001689814526639No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG31540.20665665052420945No Hit
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG28620.18752420221949506No Hit
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC25940.16996428391242843No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG3850.086.870812
CGTTTTT45550.086.173851
CGAAGGG14150.082.227442
ACGGGTA2200.079.223744
CGAGGGA12900.077.0493553
TAGGGAT23650.076.2858354
AGGGATC24350.076.234025
AGGGATT35000.076.131725
AAGGGAT47400.076.1248864
AGGGATG38900.075.868365
GAGGGAT38200.075.714884
ATAGCGG1750.075.3943561
TAGGGCA12300.075.0632254
GGGCGAT8350.074.291326
TAGGGCG2600.074.282544
ATAGGGA23300.074.197013
AGGGAAT26450.073.734895
GACCGAT1600.073.429518
AGAGGGA55350.073.3611153
AGTAGGG26150.073.1358952
Produced by FastQC (version 0.11.5)