FastQCFastQC Report
Mon 27 Feb 2023
SRR3127773.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127773.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1680575
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160420.9545542448269193No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37920.22563705874477485No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG34790.20701248084732904No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG34750.20677446707228184No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC31340.18648379274950536No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60200.085.473031
CGTAGGG6300.079.0746842
TAGCGGG8650.077.6948242
ATTGCGG1850.076.270711
AGGGCAT16850.076.143865
AGGGATG37350.075.875025
ATAGAGG13500.075.602161
TACGAGG3000.075.253771
TAGACGG2400.074.469871
TAGGGCA11650.074.227364
GGTAAGG9650.074.0840151
GAGGGAT33650.073.88274
AAGGGCA26000.073.207214
AGTAGGG27150.072.702872
ATAGCGG4150.072.533751
AGAGGGC23750.072.22733
CGAGGGA11500.071.926183
GGATGGC24150.071.809417
TAGAGGG33950.071.153372
CGAAGGG10250.071.068962
Produced by FastQC (version 0.11.5)