FastQCFastQC Report
Mon 27 Feb 2023
SRR3127772.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127772.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1269349
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117340.9244108594247917No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG31950.2517038261344989No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG31260.24626796885647684No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC29730.2342145461965149No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC26360.20766550412849422No Hit
AGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT13250.10438421584607542No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGG1600.085.3906251
CGTTTTT42650.085.055791
CGTAGGG4850.079.625112
CGAGGGA8000.077.118613
AGGGATG28400.077.111775
GTAGCGG2350.076.181231
ACGGGTA1550.075.960214
AAGGGTA10050.074.509034
ATAGGGA15300.073.875033
AACGGGT2300.073.714433
AGGGAAT17550.073.639135
GTAGGGA14550.073.47523
GCGGGAT7950.073.456874
TAGAGGG24200.073.367532
TAGGGCA8250.073.069124
ATAGCGG2200.072.809571
AGGGCAT11850.072.574795
GTAGGGC6400.072.114723
ATAGAGG9750.071.996911
GAGGGAT23950.071.773684
Produced by FastQC (version 0.11.5)